{"id":1199,"date":"2017-01-02T09:18:52","date_gmt":"2017-01-02T09:18:52","guid":{"rendered":"http:\/\/www.virologyhighlights.com\/?p=1199"},"modified":"2018-05-25T08:33:24","modified_gmt":"2018-05-25T08:33:24","slug":"the-cytoarchitecture-of-zika","status":"publish","type":"post","link":"https:\/\/www.elsevierblogs.com\/virology\/the-cytoarchitecture-of-zika\/","title":{"rendered":"The cytoarchitecture of Zika"},"content":{"rendered":"

Read the full article and view the video clips on ScienceDirect.<\/a><\/h3>\n

A comparison of Zika virus infection in human neuroblastoma and mosquito cells<\/h2>\n

Text by Danielle Offerdahl<\/em><\/p>\n

In keeping with the Rocky Mountain Laboratories tradition of tick-borne disease research, our research group has concentrated on the study of tick-borne flaviviruses.\u00a0 However, with the recent explosion of Zika virus (ZIKV), we broadened our research to include this mosquito-borne flavivirus.\u00a0 In the ongoing pandemic, ZIKV, a virus traditionally considered to cause a mild illness, has emerged as a significant neuropathogen.\u00a0 We, therefore, elected to use both a mosquito and a human neuroblastoma cell line to illuminate the impacts of ZIKV infection on these two distinct cell lines via light and electron microscopy.\u00a0 Initially, we tried using an Aedes aegypti<\/em> cell line, as this is the primary mosquito vector in the ZIKV outbreak, but were surprised to find no virus production or evidence of infection within these cells.\u00a0 Fortunately, an Aedes albopictus<\/em> cell line, C6\/36, was permissive for infection.<\/p>\n

The ZIKV-infected cell lines showed all the hallmarks of flavivirus infection. However, during our transmission electron microscopy studies, we observed the presence of \u00a020-30 nm circular structures within the 60-100 nm vesicle\/spherules that characterize flavivirus replication compartments.\u00a0 While electron dense material within these vesicles has been previously reported for other flaviviruses, no common shape had been described.\u00a0 The application of dual-tilt 3D electron tomography (ET) allowed us to show that the circular structures were, in fact, smaller spherical structures that may reflect that actual replicative intermediates of RNA and proteins.\u00a0 We performed virion and vesicle counts on the ET renderings and then calculated the numbers present in a single cell at 72 hours post-infection.\u00a0 Interestingly, the calculated values for the human neuroblastoma cells were one log higher than the counts for the mosquito cells, mimicking the one log difference seen in viral titer between the cell lines at this time point.\u00a0 The observations provided by this study create a relevant platform for future investigations the biology of ZIKV infection in both mammalian and arthropod cell lines.<\/p>\n

\"offerdahl_virology-blog_image_small\"<\/p>\n

 <\/p>\n

Image legend<\/h3>\n

3D surface rendering of membrane within Zika virus Paraiba (2015) infected human neuroblastoma cells.\u00a0 Round, hollow vesicles and smaller, solid virions are seen in abundance.<\/p>\n

Introducing the authors<\/h3>\n

\"offerdahl_virology-blog_authors\"<\/p>\n

Pictured from left to right: Marshall Bloom, Bryan Hansen, Dave Dorward, and Danielle Offerdahl.<\/p>\n

About the research<\/h3>\n

Cytoarchitecture of Zika virus infection in human neuroblastoma and Aedes albopictus cell lines<\/strong><\/a>
\nDanielle K. Offerdahl, David W. Dorward, Bryan T. Hansen, Marshall E. Bloom
\nVirology<\/em>, Volume 501, 15 January 2017, Pages 54\u201362, open access<\/p>\n","protected":false},"excerpt":{"rendered":"

Read the full article and view the video clips on ScienceDirect. A comparison of Zika virus infection in human neuroblastoma and mosquito cells Text by Danielle Offerdahl In keeping with the Rocky Mountain Laboratories tradition of tick-borne disease research, our research group has concentrated on the study of tick-borne flaviviruses.\u00a0 However, with the recent explosion Read More…<\/a><\/p>\n","protected":false},"author":1,"featured_media":1204,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5,630],"tags":[978,981,983,980,982,977,979,674,672],"class_list":["post-1199","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-highlighted-article","category-virus-host-biology","tag-aedes-albopictus","tag-bryan-t-hansen","tag-danielle-k-offerdahl","tag-david-w-dorward","tag-marshall-e-bloom","tag-neuroblastoma","tag-rocky-mountain-laboratories","tag-zika","tag-zikv"],"_links":{"self":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts\/1199","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/comments?post=1199"}],"version-history":[{"count":7,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts\/1199\/revisions"}],"predecessor-version":[{"id":1214,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts\/1199\/revisions\/1214"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/media\/1204"}],"wp:attachment":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/media?parent=1199"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/categories?post=1199"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/tags?post=1199"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}