{"id":163,"date":"2013-11-06T10:42:45","date_gmt":"2013-11-06T10:42:45","guid":{"rendered":"http:\/\/www.virologyhighlights.com\/?p=163"},"modified":"2018-05-25T08:16:44","modified_gmt":"2018-05-25T08:16:44","slug":"evolving-virus-populations-during-hepatitis-c-virus-infection","status":"publish","type":"post","link":"https:\/\/www.elsevierblogs.com\/virology\/evolving-virus-populations-during-hepatitis-c-virus-infection\/","title":{"rendered":"Evolving virus populations during hepatitis C virus infection"},"content":{"rendered":"

Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children<\/b><\/p>\n

Read the full article on ScienceDirect<\/a>.<\/p>\n

During chronic hepatitis C virus (HCV) infection, dynamic changes in viral population size and composition occur, thus facilitating viral escape to the host\u2019s immune response and establishment of persistence. Although HCV replicates primarily in the liver, extrahepatic localizations in peripheral blood mononuclear cells (PBMCs) have been described. In these sense, compartmentalization could be a mechanism contributing to viral persistence and pathogenesis. Pediatric HCV infection has a milder clinical course when compared with adult patients, but the outcome of HCV infection acquired in childhood is uncertain.<\/p>\n

In our study, we reconstructed viral evolution in plasma and PBMC samples obtained from 6 children during 3.2 to 9.6 years of follow-up. We have focused on HCV population structure and compartmentalization, which had never been evaluated before in children.<\/p>\n

Our analyses allowed dividing quasispecies into viral communities which co-evolve during chronic infection. Interestingly, these communities showed two distinct patterns of evolution, one highly divergent and another one genetically homogeneous, reflecting different virus-host interactions. We also statistically demonstrated that HCV compartmentalization in PBMC commonly exists in children. Phylogenetic networks showed highly homogeneous clusters of PBMC-derived variants, indicating some kind of restriction for HCV particles association with PBMC. Dynamic changes in quasispecies composition were different in both compartments, reflecting the PBMC pool character which contributes to viral diversity. The identification of independent lineages co-evolving in a long term infected host enables to clarify why lineages that remain undetectable for years don\u2019t go extinct but rather reappear long after. Our work highlights the significant role of compartmentalization in the maintenance of chronicity during pediatric HCV infection.<\/p>\n

The next step will be to extend the analysis to a larger region to look for viral determinants that may be associated with compartmentalization.<\/p>\n

\"Phylogenetic<\/a>
Phylogenetic networks inferred using all plasma and PBMC sequences from two patients, representative of the distinct patterns of evolution described (divergent or homogeneous). Each node corresponds to a unique sequence and the diameter of each circle refers to the frequency of that clone. Colors within the circles refer to the different time points and compartments analyzed, as indicated in the panels above each network. Viral communities are represented as rounded shape silhouettes and superimposed to each network. Temporal variations in the composition of viral communities detected in plasma and PBMC compartments are depicted in the graphics under each network. Colored areas correspond to independent communities. Black boxes over each graph indicate duration of a-IFN treatment.<\/figcaption><\/figure>\n

Introducing the authors<\/b><\/p>\n

\"mig<\/a>\"jdc<\/a>
\n(L-R) First author Mar\u00eda In\u00e9s Gismondi (Instituto Nacional de Tecnolog\u00eda Agropecuaria, Centro de Investigaci\u00f3n en Ciencias Veterinarias y Agron\u00f3micas, Buenos Aires, Argentina). First author Juan D\u00edaz Carrasco and corresponding author Mar\u00eda Victoria Preciado (Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez, Divisi\u00f3n Patolog\u00eda, Buenos Aires, Argentina).<\/p>\n

About the research<\/b><\/p>\n

Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children<\/a><\/p>\n

Virology<\/i>, Volume 447, Issues 1\u20132, December 2013, Pages 187\u2013196
\nMar\u00eda In\u00e9s Gismondi, Juan Mar\u00eda D\u00edaz Carrasco, Pamela Valva, Pablo Daniel Becker, Carlos Alberto Guzm\u00e1n, Rodolfo H\u00e9ctor Campos, Mar\u00eda Victoria Preciado<\/p>\n

Read the full article on ScienceDirect<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"

Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children Read the full article on ScienceDirect. During chronic hepatitis C virus (HCV) infection, dynamic changes in viral population size and composition occur, thus facilitating viral escape to the host\u2019s immune response and establishment of persistence. Although HCV replicates primarily Read More…<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5,634],"tags":[56,100,103,102,101],"class_list":["post-163","post","type-post","status-publish","format-standard","hentry","category-highlighted-article","category-virus-evolution","tag-hcv","tag-hepatitis-c-virus","tag-pbmc","tag-viral-evolution","tag-viral-population"],"_links":{"self":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts\/163","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/comments?post=163"}],"version-history":[{"count":1,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts\/163\/revisions"}],"predecessor-version":[{"id":167,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts\/163\/revisions\/167"}],"wp:attachment":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/media?parent=163"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/categories?post=163"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/tags?post=163"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}