{"id":272,"date":"2014-03-19T10:00:19","date_gmt":"2014-03-19T10:00:19","guid":{"rendered":"http:\/\/www.virologyhighlights.com\/?p=272"},"modified":"2018-05-25T08:17:42","modified_gmt":"2018-05-25T08:17:42","slug":"exploring-novel-biology-through-a-nematode-virus","status":"publish","type":"post","link":"https:\/\/www.elsevierblogs.com\/virology\/exploring-novel-biology-through-a-nematode-virus\/","title":{"rendered":"Exploring novel biology through a nematode virus"},"content":{"rendered":"

Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions<\/strong>
\nRead the full article on ScienceDirect.<\/a><\/p>\n

Orsay virus is the first identified virus capable of naturally infecting Caenorhabditis elegans<\/i>. Our recent discovery of Orsay virus along with two other nematode infecting viruses, Santueil and Le Blanc, enables for the first time host-virus interaction studies in the genetically tractable model organism C. elegans<\/i>.<\/p>\n

Although most closely related to nodaviruses, Orsay virus possesses a distinct genome organization with a significantly larger RNA2 segment. Genome prediction through canonical translation revealed an additional ORF in the RNA2 segment that does not share detectable homology to any known protein. As a step towards defining its function, we first sought to define how this ORF is expressed.<\/p>\n

From a series of experimental and in silico<\/i> analyses, we determined that the ORF is translated by a ribosomal frameshifting mechanism to generate a fusion with the capsid. We subsequently demonstrated that the fusion protein is likely incorporated into Orsay virions. While the function of the fusion protein remains cryptic, its presence in virions suggests that it may play a role in viral entry or other early steps of the viral life cycle. The fact that all three nematode viruses appear to encode this unique fusion protein reinforces the importance of this protein. Thus, the Orsay- C.elegans<\/i> infection model provides a wealth of opportunity to explore novel biology\u2014from the perspectives of both the host and the virus.<\/p>\n

\"A.<\/a>
A. Orsay virus is the only virus capable of naturally infecting C. elegans. B. Schema of Orsay protein expression strategy defined in this study. C. A peptide spanning the ribosomal framshifting site was detected by Mass spectrometry. D. Electron microscopy of highly purified Orsay virions.<\/figcaption><\/figure>\n

Introducing the authors
\n\"OLYMPUS<\/a>
\n<\/b>(R-L) David Wang, PhD: Associate Professor of Molecular Microbiology and Pathology&Immunology in Washington University in St. Louis School of Medicine
\nHongbing Jiang, PhD: Post-Doctoral Research Associate of Molecular Cell Biology in Washington University in St. Louis School of Medicine
\nCarl J. Franz, PhD candidate: Graduate Student of Molecular Cellbiology at Washington University in St. Louis School of Medicine
\nGuoyan Zhao, PhD: Assistant Professor of Pathology&Immunology in Washington University in St. Louis School of Medicine<\/p>\n

About the research<\/b><\/p>\n

Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions<\/a>
\n<\/b>Virology<\/i>, Volumes 450\u2013451, February 2014, Pages 213\u2013221
\nHongbing Jiang, Carl J. Franz, Guang Wu, Hilary Renshaw, Guoyan Zhao, Andrew E. Firth, David Wang<\/p>\n

Read the full article on ScienceDirect.<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions Read the full article on ScienceDirect. Orsay virus is the first identified virus capable of naturally infecting Caenorhabditis elegans. Our recent discovery of Orsay virus along with two other nematode infecting viruses, Santueil and Le Blanc, enables for the first Read More…<\/a><\/p>\n","protected":false},"author":1,"featured_media":274,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5,629],"tags":[159,161,69,160,162],"class_list":["post-272","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-highlighted-article","category-virus-replication","tag-caenorhabditis-elegans","tag-host-virus-interaction","tag-orf","tag-orsay-virus","tag-rna2"],"_links":{"self":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts\/272","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/comments?post=272"}],"version-history":[{"count":1,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts\/272\/revisions"}],"predecessor-version":[{"id":275,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/posts\/272\/revisions\/275"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/media\/274"}],"wp:attachment":[{"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/media?parent=272"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/categories?post=272"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.elsevierblogs.com\/virology\/wp-json\/wp\/v2\/tags?post=272"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}